CoLocalizer Express

Developer: CoLocalization Research Software

Current Version: 1.7.1

Last Updated: 4 years ago

Download Size: 2.9 MB - Download

Description:

CoLocalizer Express is a biomedical scientific application for quantification of colocalization in fluorescence microscopy images. It helps to define precise locations of proteins and nucleic acids in tissues and cells and understand functional significance of protein-protein interactions. CoLocalizer is used by the leading research laboratories worldwide.

CoLocalizer Express can open image files in all major file formats, such as TIFF, JPEG, PICT, BMP, etc. It also supports proprietary file formats from all major microscope manufacturers and a biological image data standard.

Quantification of colocalization using CoLocalizer Express is based on calculation of the following coefficients: Pearson's correlation coefficient (Rr), overlap coefficient according to Manders (R), overlap coefficients k1 and k2, colocalization coefficients m1 and m2, and colocalization coefficients M1 and M2. Obtained results can be saved in Excel file format for further analysis. For more information on the theory of quantitative colocalization analysis, visit our website: http://www.colocalizer.com/probasics.html

Reliability of calculations is ensured by using the background correction tool. Using this tool, pixels with intensities potentially contributing to false counts are effectively removed from the image.

To get more information, read the FAQs, watch video tutorials, and more visit our website: http://www.colocalizer.com/

To view CoLocalizer-based protocol, view the paper published by Nature Protocols: http://www.nature.com/nprot/journal/v6/n10/abs/nprot.2011.384.html

For further inquiries, contact us: [email protected]

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Release Notes:

- Enabled sandboxing.
- Added Histogram functionality.
- Show Info window has been transformed into a panel.
- Show Info panel now always appear on top of the main application window.
- Values of the Show Info panel are now selectable.
- Show Info panel now indicates what kind of background correction was applied to the image.
- Background correction window now always appear on top of the main application window.
- Added proper types of pointers when selecting and moving scatter gram ROI.
- Added the help button to the warning about background correction step when calculating coefficients for the scatter gram ROI.
- Improved text of the background correction warning.
- Improved layout of the About Box, updated its contents.
- Improved layout of the main application window.
- Improved Mac OS X 10.8 (Mountain Lion) support.
- Now uses a crosshair cursor over the histogram.
- Fixed a bug when changes of ROI settings in the preferences were clearing selected scatter gram ROI.
- Fixed a bug when changes of ROI shapes in the scatter gram did not reset scatter gram values.
- Fixed a bug when changes of the selection of pair of channels did not refresh scatter gram values.
- Fixed a bug when scatter gram ROI was selectable with no image opened.
- Fixed a bug when pixel resolution was shown erroneously for some images.
- Fixed an issue of Level and Count values for All channels in the histogram not displaying.
- Fixed a bug when image depth was shown incorrectly for some images.
- Fixed an issue of Level and Count values for All channels in the histogram not displaying.
- Fixed a bug when image depth was shown incorrectly for some images.
- Fixed a refresh bug in the Show Info panel on image loading.
- Fixed a bug when cursor was not updated with the histogram.
- Fixed a bug when main page of the built-in Help may have displayed incorrectly.
- Exported files are now saved to a folder.
- Updated contents of the built-in Help.

This update is recommended for all users of CoLocalizer Express.

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